Germany & USA: PIAS1 is not suitable as a therapeutic agent for urothelial cancer
Gene expression analysis using Mic qPCR Cycler on PIAS1 demonstrates that it plays a minor role in cell survival and DNA repair within urothelial cancer cells in vitro and should not be used as a therapeutic agent.
USA: Sexual transmission of Zika virus typically only occurs in acutely infected mice.
Quantitative RT-qPCR was used to determine the possibility of sexual transmission of zika virus from infected males in chronic stages of disease. The expression levels of ZIKV RNA in intravaginal inoculated and subcutaneous inoculation groups of female and male mice were evaluated.
Italy: Infrapatellar Fat Pad stem cells exert incomplete protective activity from OA inflammation
The study employed techniques such as gene expression, performed on the MIC, to determine the structural and functional characterisation of osteoarthritic infrapatellar fat pad stem cells and their role in osteoarthritic inflammation.
South Korea: Modular Chip for Bacterial DNA Extraction
A modular chip was developed to simplify and reduce the cost of bacterial DNA extraction. The chip demonstrated high efficiency and reproducibility with similar performance to conventional methods. It can potentially be used for Point-of-Care testing.
Italy: Infrapatellar Fat Pad stem cells exert incomplete protective activity from OA inflammation
The study employed techniques, such as gene expression performed on the MIC, to determine the structural and functional characterization of osteoarthritic infrapatellar fat pad stem cells and their role in osteoarthritic inflammation.
Poland: qPCR for the detection of Legionella spp. and E. Coli in hot tap water systems
In this study, L. spp., L. pneumophila, and E. coli bacteria were quantified from total DNA extracts of hot tap water samples using the MIC qPCR platform with TaqMan probes. The obtained results prove the presence of these bacteria in hot tap water installations in the majority of the examined buildings.
Finland: Molecular point-of-care testing for rapid diagnosis of common colds occurring in elite athletes
Molecular point-of-care testing (POCT) consists of a multiplex qPCR for rapid diagnosis of the aetiological agent causing the common cold. It allows for effective treatment and enhance timely isolation to decrease viral transmission.
Iran & USA: GO/Au nanocomposite enhances amplification in PCR systems
The effect of GO/Au nanocomposite was studied on end-point and real-time PCR (performed on the Mic Real-Time Cycler) employed for amplification of human GAPDH gene. The results showed that the GO/Au nanocomposite can improve both end-point and real time PCR methods at optimum concentrations, possibly through providing increased thermal convection by the GO surface as well as the Au nanostructures.
USA & China: Noninflammatory changes in Microglia contribute to epileptogenesis
The effects of electroconvulsive shock on microglia was measured by mRNA expression analysis, using two-step RT-qPCR performed on the Mic Real-Time Cycler. The results showed physiological changes in microglia but no neural injury, which may be relevant to electroconvulsive therapy.
Hungary: Severe kidney disease in Malaysian broiler chickens
A RT-qPCR, performed on the Mic Real-Time Cycler, was used to validate the identification of the etiological agent causing severe kidney disease in broiler chickens. The virus detected was found to be the virus Kedah fatal kidney syndrome virus (KFKSV).
USA & China: Rapid diagnostic kit for accurate assessment of antifungal resistance of C. auris
A rapid diagnostic kit was developed for accurate and rapid assessment of FKS1-associated echinocandin resistance and ERG11-associated azole resistance in C. auris. The diagnostic platform used a duplex asymmetric PCR in conjunction with molecular beacon probe-based melting curve analysis that was performed on the Mic Real-Time PCR cycler.
Switzerland: ChIP assay for study of specific-nuclear signaling factors
In this study, a TF-ChIP protocol was developed to study specific nuclear signalling factors in cancers. The protocol produces high quality DNA output and can be reproduced using both fresh and fresh-frozen tissues, which is important in a clinical setting.
Canada & USA: Pathogenesis of Phytophthora sojae on soybean
Gene expression of the pathogenic genes in Phytophthora sojae were studied to further understand the gene-for-gene interaction between the pathogen and resistant soybean genes. In this study, a RT-qPCR was performed on the MIC and found that genomic signatures can predict phenotype compatibility between these genes.
RT-qPCR experiments were performed on the MIC to identify the presence of five plant food-derived miRNAs that are present in human breast milk. Two of these miRNAs were found to be in exosomes and may influence crucial biological pathways in infants.
Switzerland: Metabolic regulation in Lactobacillus paracasei FAM18149
Next-generation sequencing and RT-qPCR performed on the MIC were utilized in this study to understand the metabolic pathway of sulfur-containing amino acids, cysteine and methionine, in Lactobacillus paracasei.
Switzerland: Effector molecules of pathogens prevent disease resistance in plants
In this study, the expression levels of the effecter molecule, RxLR24, present in Phytopthora pathogens were quantitated on the MIC. It was found that expression occurred early in the interaction between the pathogen and the plant and inhibits disease resistance in plants.
Identifying protective feline immunodeficiency virus (FIV) peptides that are evolutionarily conserved with human immunodeficiency virus (HIV) is useful in identifying protective HIV peptides that are required for successful HIV vaccines in humans.
In this study, a RT-qPCR on the MIC was used to analyse the expression of Acyl-CoA synthetase-4 (ACSL4), an enzyme involved in therapy resistance, and ATP-binding cassette (ABC) transporters, which detect and eject anti-cancer drugs from cells. It was concluded that ACSL4 can be used as a regulator of ABC transporters and increase the success of drug therapies in breast cancer.
mRNA expression levels of neural stem cells (NSC) with FAD treatment was studied using RT-qPCR performed on the MIC. It was found that FAD suppresses the notch pathway of NSC, allowing excessive proliferation and differentiation.
The viral RNA loads of wobbly possum disease virus in experimentally infected possums were studied using RT-qPCR on the MIC. The study found high levels of RNA in lymph nodes, spleens and livers as well as moderate levels in serum and saliva of the infected possums.
This study detected Zika virus in all components of the male reproductive tract by quantitative RT-PCR performed on the MIC. All components of the male reproductive tract were capable of sexually transmitting the Zika virus to naïve females.
This study discovered the presence of the dacZ gene in an archaeal species, indicating production of c-di-AMP. The expression of dacZ was observed using qPCR, performed on the MIC, and found it constantly expressed at different growth phases. This provides evidence that the gene is essential in this archaeal species and thus, indicating c-di-AMP is also essential.
A quantitative PCR using the MIC Real-Time Cycler was used to validate an acute Toxoplasma gondii assay. 12.3% of 276 pregnant women in Salmas, North West of Iran, were identified as positive for acute T. gondii.
China, Australia and USA: Host-derived extracellular exosomes act as a defence mechanism during Haemonchus contortus parasitic infections.
RNA-sequencing of transcriptome responses in resistant and susceptible sheep flocks reveal 38 genes differentially expressed. The expression of these genes was further confirmed using qRT-PCR, performed on the MIC Real-Time Cycler.
USA, China & Canada: Zika virus polyclonal antibodies
Quantification of ZIKV infection, using the MIC Real-Time Cycler, on non-treated and treated hamsters with SAB-155 antibody revealed sufficient protection against the virus, even 4.5 days post infection.
Australia: Epidemiology of Toxoplasma gondii and the risk factor associated for Eastern Barred Bandicoot relocation on Phillip Island.
A qPCR, using MIC Real-Time Cycler, was used to study the prevalence of Toxoplasma gondii in feral cats and rabbits on Phillip Island to understand the associated risks for the introduction of the Eastern Barred Bandicoot.
Germany and Tunisia: Brucella in cerebrospinal fluid
A rapid real-time PCR assays using a Magnetic Induction Cycler (MIC) was established and validated for Brucella. Cerebrospinal fluid were obtained from patients diagnosed with encephalitis and meningitis in Tunisia hospitals between 2012-2017.
Korea: Protective effect of hydrolysates from seahorse on Atherosclerosis
After determining the most promising hydrolysate for an anti-atherosclerotic agent using seahorse, a qPCR using MIC was conducted to determine the effects of the hydrolysate on the expression of apoptosis-related genes.
Iraq: micro-RNA levels in follicular fluid indicate embryo quality
Real time PCR using MIC was used to determine if there is an association between miRNA-320 levels in follicular fluid and embryo quality in IVF-ISCI procedures. The miRNA levels in differing grades of embryo qualities was compared and an association was found with higher grade embryos containing more miRNA-320.
Australia, Thailand, Switzerland, Cambodia: Multiplex qPCR for Hookworms
The authors developed a multiplex qPCR for simultaneous detection of three Hookworm species. The ability to quantify results allows this newly developed assay to be used for monitoring and efficacy programs.
New Zealand: Selenium compounds as therapeutic agents in cancer patients
The study compared three oral selenium compounds to determine the dose and form that is the most effective and is safe to use as a therapeutic agent in cancer patients. A qPCR was used to determine the genotoxicity of the compounds.